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. Author manuscript; available in PMC: 2019 Apr 25.
Published in final edited form as: Chem Rev. 2018 Jan 24;118(8):4120–4155. doi: 10.1021/acs.chemrev.7b00519

Figure 2. Photo-physical properties of fluorescent molecules and different SM excitation and emission methods.

Figure 2

(a) Jabłoński Diagram: A photo-physical description of fluorescence. S0, S1-Sn, and T1-Tn represent the singlet ground state, singlet excited states and triplet excited states respectively. Schematic representations of excitation and emission pathway of different SM microscopes: (b) confocal, (c) objective type TIRF and (d) prism type TIRF.