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. 2018 Apr 26;14:33. doi: 10.1186/s13007-018-0301-z

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Profile of selected lignans and their precursor metabolites. 6-week-old plants were processed for relative quantification analysis in a UHPLC-QTOF MS. The metabolite concentration of each metabolite was normalized by using the chromatographic area for each compound with the dry weight of the corresponding sample. The third and fourth leaves of 3 individual plants 6-weeks-old was harvested and pooled for each genotype. Box plots represent the means for three independent experiments with two technical replicates. Different letters indicate statistically significant differences (ANOVA, Fisher’s least significant difference (LSD) test, p > 0.05, n = 6)