Figure 1.

Enrichment and Proteomic Characterization of hESC-Derived ISL1⁺ Progenitors

(A) Schematic representation of the differentiation protocol.

(B) Real-time qRT-PCR for ISL1 expression during cardiac differentiation of rH5-isl1-Hygro. n = 3.

(C) Antibiotic treatment paradigm for enrichment of ISL1⁺ cells.

(D) Immunofluorescence staining and flow cytometry of hESCs at day 8 of differentiation with or without antibiotic treatment for ISL1.

(E) Membrane proteins that were ≥1.5-fold differentially expressed (n = 3 independent experiments) between antibiotic-treated cells versus untreated cells. AA, activin A.

Data are mean ± SEM. ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001. Scale bar, 75 μm.