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. Author manuscript; available in PMC: 2018 Apr 27.
Published in final edited form as: Cell Rep. 2018 Apr 3;23(1):156–171. doi: 10.1016/j.celrep.2018.03.015

Figure 5. prg-1 Germline Arm Phenotypes and Sterility Can Be Suppressed.

Figure 5

(A) Sterile day 1 prg-1(n4357) cep-1(gk138) animals were scored for continued sterility from days 2 through 6. n represents total worms scored on that day that were previously sterile.

(B) Germline phenotypes of sterile germlines in day 1, day 2, and day 3 prg-1 daf-16 mutants.

(C) Effects of apoptosis and necrotic cell death pathways on sterile germline phenotypes of prg-1. For comparison, we include wild-type, prg-1, and prg-1 cep-1 data that are the same data as shown in Figure 1D. p values were determined using pairwise chi-square tests with Bonferroni correction that can be found in Table S1.

(D) Sterile day 5 prg-1 mutants on either standard laboratory plate NGM plates with OP50 E. coli (OP50:NGM) or on RNAi plates seeded with various bacteria relevant to generating dsRNA (HT115:RNAi clone; EV is empty vector) scored for percent fertility 48 hr after treatment (day 7) *p < 0.05, Fisher’s exact test with Bonferroni correction.

(E) Sterile day 5 prg-1 mutants on OP50 bacteria on standard RNAi plate condition (OP50:RNAi) scored for percent fertility 48 hr after treatment (day 7). Data combined from minimum of three independent experiments; n represents total worms (A, D, and E) or germline arms (B and C) scored.