Figure 7.

Effects of ABA and cytokinin on ELIP accumulation. After 7 d of growth barley leaf segments were floated for 24 h on either tap water (H₂O) or 2 mm of cytokinin (cytokinin) or 1 mm of ABA (ABA). Following these pre-incubations the segments were either subjected for 4 h to HL of 2,500 μmol m⁻² s⁻¹ (2500), 1,250 μmol m⁻² s⁻¹ (1250), or 625 μmol m⁻² s⁻¹ (625) or further incubated for 4 h at LL conditions of 100 μmol m⁻² s⁻¹ (100) or directly frozen in liquid nitrogen (100*). Fifteen μg of protein per lane were analyzed by western blot probed with anti-ELIP after SDS-PAGE (15% [w/v] gels). See also legend to Figure 2 B.