Fig. 4.
CF33 is safe in mice and causes regression of both injected and non-injected distant tumors at a low dose in PANC-1 and MIA PaCa-2 xenograft models. Female athymic nude mice were implanted in the bilateral flank with either PANC-1 or MIA PaCa-2 and a single injection of 103 †PFU of CF33 for PANC-1 and 105 PFU for MIA PaCa-2 were administered intra-tumorally in the left tumor. a Percent change in tumor volume in virus-injected PANC-1 tumors (a) and MIA PaCa-2 tumors b was significantly lower compared to PBS‡-injected tumors. c Percent change in the non-injected distant tumors in PANC-1 was significantly smaller with CF33 compared to PBS-injected controls. d Toxicity in PANC-1 xenograft bearing mice was determined by percent change in net body weight. No significant difference in weight change was observed between the virus-treated mice and the PBS-treated mice, suggesting that the dose of virus used in this study is safe in immune-compromised mice (*p < 0.05. **p < 0.01. ****p < 0.0001. ns, not significant. †PFU, plaque forming units. ‡PBS, phosphate buffered saline)