Fig. 5.

Virus-encoded luciferase activity correlates with tumor regression over time and increasing viral titers in the non-injected tumors. To ensure luciferase visualization, CF33-Fluc was administered at 10⁴ PFU to PANC-1 xenograft-bearing immune-compromised mice. a Relative luciferase activity decreased in the injected tumors and increased in the non-injected distant tumor over time. b Percent change in CF33-Fluc-injected tumor size and luciferase activity were correlated (R² = 0.879). c CF33-Fluc enabled real time in vivo monitoring of viral replication at days 3, 13 and 27 and showed replication in both the injected and non-injected distant tumors. d CF33-Fluc titers in the injected tumors are at least 4 logs higher than in other organs, indicating early preferential replication in tumors. Virus titers in the non-injected tumors increased over time, which correlates to luciferase activity