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. 2018 Apr 25;12:23. doi: 10.1186/s40246-018-0155-5

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Cellular characterization of p.Q118dup BMP6 protein in CHO cells. a Representative immunofluorescence image of stably transfected CHO cells. The HA tag on the N-terminus of the pro-peptide, and the V5 tag on the C-terminus of the mature protein co-localize, and no difference in localization of the wildtype (WT) and p.Q118dup variant of BMP6 was observed. b Western blot identifying the full-length (FL) and mature BMP6 proteins in cell lysate (CP) and conditioned media (M) from untransfected (UT), wildtype (WT), and p.Q118dup (Q118dup) BMP6 transfected CHO cells. c Quantification of Western blot shown in b (n = 3). The ratio of mature proteins in conditioned media (M) to cell lysate (CP) shows no difference in protein secretion between WT and p.Q118dup BMP6 transfected cells