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. 2018 Apr 25;13:34. doi: 10.1186/s13019-018-0720-y

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Valve characterization: methods. a: macrophotograph of a DPV. b: microphotograph of NPV after trichrome staining illustrating histological assessment of the valves in 3 zones:1: the muscle/2: the leaflet/3: the wall. Scal barr: 1 mm. For histological evaluation, the explanted valves were sectioned in six segments (c) to analyze the three zones (d)