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. 2018 Apr 25;14(4):e1007325. doi: 10.1371/journal.pgen.1007325

Fig 6. The expression of dominant negative NUP82 and its impact on Ty1 mobility.

Fig 6

(A) Left. A linear domain diagram of Nup82pDFY-LILLF and derived deletion mutants [Nup82p(433–713) and Nup82pDFY-LILLF(1–458)]. Asterisks mark the mutations that decouple Nup82p from the nuclear pore complex. Right. Western blot analysis to detect the expression of FLAG-tagged Nup82pDFY-LILLF and its derivatives, compared to the expression of a control protein (Met17p) in the wild-type background (*Met17p degradation products). The effect of Nup82pDFY-LILLF expression on (B) Ty1 mobility, (C) doubling time in a liquid medium and (D) the nuclear import of the reporter protein LexA-MBP-Gal4(AD), relative to the expression of MET17 (error bars: standard error, n>3; **Tukey–Kramer method, p<0.05).