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. Author manuscript; available in PMC: 2018 Apr 27.
Published in final edited form as: Lab Invest. 2014 Jul 28;94(9):1030–1041. doi: 10.1038/labinvest.2014.85

Figure 2.

Figure 2

Fibrogenic gene expression in cultured ocular fibroblasts. (a) Adding exogenous transforming growth factor-β1 (TGF-β1) upregulated transient receptor potential ankyrin 1 (TRPA1) mRNA expression in wild-type (WT) mice ocular fibroblasts. (b and c) Loss of TRPA1 reduced the basal mRNA expression level of TGF-β1 and interleukin (IL-6) in ocular fibroblasts, and the degree of upregulation of these genes by exogenous TGF-β1 was also attenuated by the loss of TRPA1. (d) There was no difference in the basal expression level of α-smooth muscle actin (α-SMA) mRNA in between cultured WT and TRPA1-null (KO) ocular fibroblasts. In cultured WT ocular fibroblasts, the expression of α-SMA was markedly upregulated by adding exogenous TGF-β1, which was abolished with the loss of the TRPA1 gene in the fibroblasts. *P<0.05, bar, mean±s.e.m.