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. 2018 Mar 1;10(3):693–702. doi: 10.1016/j.stemcr.2018.01.025

Figure 2.

Figure 2

Screening of Niche Factors Using HLI Algorithm Demonstrates Effect of Laminin 411 in i-Heps

(A) The HLI algorithm (y axis) was used to screen for the effects of 58 different hepatocyte niche factors (x axis) on i-Heps 7 days after plating. Control line (red) is the control threshold based on culture with collagen-1.

(B) Revalidation of the eight hits from the first round of screening.

(C) Relative gene expression of i-Heps cultured on Laminin 411 (middle bars) compared with collagen-1 (left bars) with expression levels in freshly isolated adult hepatocytes (AH) as control (right bars). n = 3 (different i-Hep cell lines and independent experiments); error bars show mean ± SD; ∗p < 0.05, ∗∗∗p < 0.01.

(D) Immunofluorescence staining for albumin (red, left), DAPI (blue, left middle) and merge (middle right), of i-Heps cultured on Laminin 411 (top) versus collagen-1 (bottom); cell morphology is shown on far right (10× magnification; scale bar, 100 μm). Images shown represent n = 3 different biological replicates and independent experiments. Scale bar, 200 μm.