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. 2018 Mar 1;10(3):693–702. doi: 10.1016/j.stemcr.2018.01.025

Figure 3.

Figure 3

Laminin 411 Is a Physiologically Relevant Niche Factor in Fetal Liver Development

(A) Morphology (20×) of fetal hepatocytes cultured on collagen-1 (top) versus Laminin 411 (bottom) at 2 (left) and 5 (middle) days post plating (scale bar, 200 μm). Immunofluorescence staining (right) for albumin (red) plus DAPI (blue) at 5 days post plating (scale bar, 100 μm).

(B) Number of albumin-expressing fetal hepatocytes identified by HLI algorithm (y axis), cultured on Laminin 411 versus collagen (x axis) at day 5. n = 3 different biological samples and independent experiments; data presented as mean ± SD; ∗p < 0.05.

(C) Relative gene expression (y axis) of fetal hepatocytes cultured for 7 days on Laminin 411 (middle bars) versus collagen 1 (left bars) compared with adult liver (right bars). n = 3 (different biological samples and independent experiments); data presented as mean ± SD; ∗p < 0.05.

(D) ECM gene expression heatmap comparing iPSC-derived endoderm (left) with adult (middle) and fetal (right) liver.

(E) RNA in situ hybridization for LAMA4 on a 16-pcw liver slide (left) (40× magnification; scale bar, 50 μm). Detail of the red dots expanded in the square compared with adult liver (right).