Table II.

The effect of NADP⁺ and NADPH addition on ROS production and the HR

		ROS Production in the Incompatible Interaction			Percentage Cell Viability
		HO2./O2−a		H2O2b	Control	Incompatible interaction
		XTT	Cyt c
		10−9 mol 0.1 g−1 cells			%
±NADP+ (1 mm)	Nil	48.0 ± 9.1	–	34.8 ± 3	88.7 ± 1.2	39.0 ± 1.2
	NADP+	24.6 ± 4.9	–	15.2 ± 1.1	80.9 ± 0.6	44.6 ± 1.5
±NADPH (1 mm)	Nil	—	14.6 ± 1.2	34.8 ± 3	84.6 ± 1.8	42.5 ± 0.8
	NADPH	—	19.7 ± 0.7	67.3 ± 3.8	87.0 ± 3.5	32.9 ± 2.4

One millimolar NADP⁺ or 1 mM NADPH was added at the time of Pn 4974 zoospore (incompatible) or water (control) addition to NC cells. Data represent means ± se of n = 12 from three experiments.

^a Determined from Mn(III)desferal inhibitable XTT or Cyt c reduction. 

^b Based on O₂ evolution.