2D and 3D Culture of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes
(A) Schematic depiction of experimental setup.
(B and C) Bright-field image of representative 2D and 3D hiPSC after 3 weeks; still frame from Movie S1.
(D) Force development of 3D hiPSC-CMs. Black frame indicates time window for functional analyses.
(E) qPCR results of 2D and 3D hiPSC-CMs for cardiomyocyte (MYL3, MYL4, cTNT1), fibroblast (vimentin, POSTN), and endothelial (CD31) markers.
(F and G) Transmission electron microscopy (TEM) pictures in 2D compared with 3D hiPSC-CMs (black arrow, mitochondria; white arrow, degenerated mitochondria). (F′) and (G′) are blow-ups of dashed boxes in (F) and (G) (open arrowheads: glycogen granules); samples prepared from one experiment.
n denotes biological replicates/independent experiments of both control cell lines; error bars show means ± SEM. Related data are depicted in Figure S1 and Movie S1.