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. 2018 Mar 1;10(3):834–847. doi: 10.1016/j.stemcr.2018.01.039

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© 2018 The Author(s)

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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2D and 3D Culture of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes

(A) Schematic depiction of experimental setup.

(B and C) Bright-field image of representative 2D and 3D hiPSC after 3 weeks; still frame from Movie S1.

(D) Force development of 3D hiPSC-CMs. Black frame indicates time window for functional analyses.

(E) qPCR results of 2D and 3D hiPSC-CMs for cardiomyocyte (MYL3, MYL4, cTNT1), fibroblast (vimentin, POSTN), and endothelial (CD31) markers.

(F and G) Transmission electron microscopy (TEM) pictures in 2D compared with 3D hiPSC-CMs (black arrow, mitochondria; white arrow, degenerated mitochondria). (F′) and (G′) are blow-ups of dashed boxes in (F) and (G) (open arrowheads: glycogen granules); samples prepared from one experiment.

n denotes biological replicates/independent experiments of both control cell lines; error bars show means ± SEM. Related data are depicted in Figure S1 and Movie S1.