Metabolic Ability of 2D CMs and 3D CMs
(A) Beating rate for hiPSC-CMs of both control cell lines in 2D.
(B) Beating rate and contractile force for hiPSC-CMs of both control cell lines in 3D in feeding medium containing only one energy source for 20 hr, 1 mM fatty acid, 1 mM lactate, 5 mM glucose, or no energy substrate.
(C–E) Normalized force development (C) and original recordings (D and E) of 3D hiPSC-CMs during 100 min of hypoxia treatment (gray box) and reoxygenation.
n denotes biological replicates from three independent experiments of both control cell lines; error bars show means ± SEM. Related data are depicted in Figure S5 and Movie S2.