Skip to main content
. 2018 Mar 1;10(3):834–847. doi: 10.1016/j.stemcr.2018.01.039

Figure 7.

Figure 7

Analysis of Mitochondrial Biogenesis after Pharmacological Reduction of Force

(A) Original recordings of EHTs ± myosin II inhibitor blebbistatin (300 nM) for 7 days from day 14 to day 21.

(B) Comparison of contractility parameter of EHTs incubated ± blebbistatin (300 nM) on day 21.

(C) PCR amplification of genomic DNA for Mt-ND1/2, normalized to the nuclear encoded gene actin of 3D ± blebbistatin.

(D) MitoTracker Green FM FACS of dissociated 3D hiPSC-CMs ± blebbistatin (300 nM).

(E) PCR amplification of genomic DNA for Mt-ND1/2, normalized to the nuclear encoded gene actin of 3D ± BTS.

n denotes biological replicates from three independent experiments. ∗p < 0.05 versus 2D (two-tailed unpaired t test); error bars show means ± SEM. Related raw data of individual experiments are depicted in Figure S7.