Fig 7. Frequencies of HLA-B*08:01-restricted HHV-6-specific T cells in peripheral blood.

PBMCs from eight healthy adult HLA-B*08:01-positive donors were stained with thirteen HLA-B*08:01/peptide multimers (dextramers) carrying HHV-6 peptides as indicated. An HLA-B*08:01 dextramer carrying the EBV epitope RAK served as a control. (A) Examples of dextramer staining for two donors and six dextramers. (B) Frequencies of multimer-staining cells in eight healthy donors. Donors were known to be HHV-6-seropositive, except donor 9, whose serostatus was not known. Only four donors could be stained with RAK; donor 3 was not stained with TNK and MAR. A median of 7×10⁵ cells was stained for FACS analysis. (C) Number per donor of HHV-6B/6A multimer-staining populations that exceeded 0.01% of CD8 T cells. (D) For each HHV-6 epitope, the proportion of donors is shown who had multimer-staining populations that exceeded 0.01% of CD8 T cells.