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. 2018 Apr 26;13(4):e0196698. doi: 10.1371/journal.pone.0196698

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© 2018 Dhumale et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 2 — (A, C, E) Coronal sections from the brains of E13 to E17 embryos with the indicated genotype were stained with antibodies for Tbr1⁺ (marker for L5/6 and subplate; green) or Ctip2⁺ (L4–6; red) and Hoechst 33342 (blue). (B, D, F) The number of Tbr1⁺ (B, F) and Ctip2⁺ cells (D, F) in the cortex (B, D) and hippocampus (F, E17) normalized to an area of 10⁴ μm² is shown for the Sada^+/-;Sadb^+/- (magenta) and Sada^-/-;Sadb^-/- cortex (green). Values are means ± s.e.m., n = 3 different embryos, two-way ANOVA compared to heterozygous control; *, p<0.05; **, p<0.01, ***, p<0.001. The scale bars are 50 μm.