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. 2018 Apr 26;13(4):e0196434. doi: 10.1371/journal.pone.0196434

Table 2. Targeted regions of amplification for the NGS assay.

Gene Hotspot Transcript Gene Hotspot Transcript
AKT1 E17 NM_001014432.1 IDH1 R132 NM_005896.3
ALK T1151, L1152, C1156, F1174, L1196, G1269, R1275 NM_004304.4 IDH2 R140, R172 NM_002168.3
AR S741, W742, H875, Q876, T878 NM_000044.3 JAK1 V658, S703 NM_002227.2
BRAF Q201, G466, G469, Y472, D594, G596, L597, V600 NM_004333.4 KIT D816, D820, N822, Y823, exons 11, 13 NM_000222.2
CDKN2A R58 NM_000077.4 KRAS G12, G13, Q61, K117, A146 NM_004985.4
CTNNB1 S37, T41, S45 NM_001904.3 MAP2K1 Q56, K57, K59, D67, P387 NM_002755.3
EGFR exons 18, 19, 20, 21 NM_005228.3 MAP2K2 F57, Q60, K61, L119 NM_030662.3
ERBB2 G309, S310 NM_004448.3 MET Y1253, exons 13, 18 NM_001127500.1
ESR1 V534, P535, L536, Y537, D538 NM_001122742.1 NRAS G12, G13, Q61, K117, A146 NM_002524.4
FGFR1 N546, K656 NM_023110.2 PDGFRA D842 NM_006206.4
FGFR2 S252, P253, N549, K659 NM_000141.4 PIK3CA E542, E545, Q546, D549, M1043, N1044, A1046, H1047, G1049 NM_006218.2
GNA11 Q209 NM_00267.4 PTEN R130, R173, R233 NM_000314.4
GNAQ Q209 NM_002072.4 RET C634, M918 NM_020975.4
GNAS R201 NM_000516.4 STK11 Q37, P281, F354 NM_000455.4
HRAS G12, G13, Q61 NM_005343.2