Skip to main content
PMC home page
Japanese Journal of Cancer Research : Gann logoLink to Japanese Journal of Cancer Research : Gann
. 1994 Sep;85(9):918–926. doi: 10.1111/j.1349-7006.1994.tb02969.x

Monoclonal Antibody against PRADl/Cyclin Dl Stains Nuclei of Tumor Cells with Translocation or Amplification at BCL‐1 Locus

Shogo Banno 1,2, Kazuhiro Yoshikawa 3,4, Shigeo Nakamura 5, Kazuhito Yamamoto 1, Tsutomu Seito 6, Masakazu Nitta 2, Toshitada Takahashi 3, Ryuzo Ueda 1, Masao Seto 1,✉
PMCID: PMC5919590  PMID: 7961120

Abstract

Mouse monoclonal antibodies were produced against the bacterial product encoded by human PRAD1/ cyclin Dl gene, which is known to be involved in tumors with translocation or amplification at BCL‐1 locus of 11ql3. The immunizing antigens used were GST‐PRAD1 and T7 gene 10‐PRAD1 fusion products. Four antibodies were reactive with both PRAD1 fusion products and cell lysates of B‐cell tumor cell lines with t(ll;14)(ql3;q32) and a breast cancer cell line with 11ql3 amplification, on immunoblotting. An immunofluorescence study showed that only one of them stained nuclei of cells with 11q13 abnormalities. Since this antibody proved applicable for conventional paraffin‐embedded tissue sections, immunohistologic staining of various lymphoma tissues was performed. Eight of 11 mantle cell lymphomas showed intermediate to strong positivity and 6 of the positive cases demonstrated characteristic staining patterns that were either predominantly nuclear or both nuclear and cytoplasmic. The nuclear staining pattern was not observed with other types of lymphoma and thus may correlate with PRAD1 mRNA overexpression.

Keywords: PRAD1, Cyclin D1, BCL‐1, Chromosomal translocation, Lymphoma

Full Text

The Full Text of this article is available as a PDF (885.2 KB).

REFERENCES

  • 1. ) Tsujimoto , Y. , Yunis , J. , Onorato‐Showe , L. , Erikson , J. , Nowell , P. C. and Croce , C. M.Molecular cloning of the chromosomal breakpoint of B‐cell lymphomas and leukemias with the t(11; 14) chromosome translocation . Science , 224 , 1403 – 1406 ( 1984. ). [DOI] [PubMed] [Google Scholar]
  • 2. ) Ali , I. U. , Merlo , G. , Callahan , R. and Lindereau , R.The amplification unit on chromosome 11q13 in aggressive primary human breast tumors entails the bcl‐1, int‐2 and for loci . Oncogene , 4 , 89 – 92 ( 1989. ). [PubMed] [Google Scholar]
  • 3. ) Theillet , C. , Le Roy , X. , De Lapeyriere , O. , Grosgeorges , J. , Adnane , J. , Raynaud , S. D. , Simony‐Lafontaine , J. , Goldfard , M. , Escot , C. , Birnbaum , D. and Gaundray , P.Amplification of FGF‐related genes in human tumors: possible involvement of HST in breast carcinomas . Oncogene , 4 , 915 – 922 ( 1989. ). [PubMed] [Google Scholar]
  • 4. ) Proctor , A. J. , Coombs , L. M. , Cairns , J. P. and Knowles , M. A.Amplification at chromosome Ilql3 in transitional cell tumours of the bladder . Oncogene , 6 , 789 – 795 ( 1991. ). [PubMed] [Google Scholar]
  • 5. ) Tsujimoto , Y. , Jafle , E. , Cossman , J. , Gorham , J. , Nowell , P. C. and Croce , C. M.Clustering of breakpoints on chromosome 11 in human B‐cell neoplasmas with the t(11;14) chromosome translocation . Nature , 315 , 340 – 343 ( 1985. ). [DOI] [PubMed] [Google Scholar]
  • 6. ) Meeker , T. C. , Grimaldi , J. C. , O'Rourke , R. , Louie , E. , Juliusson , G. and Einhorn , S.An additional breakpoint region in the BCL‐1 locus associated with the t(11;14) (q13;q32) translocation of B‐lymphocytic malignancy . Blood , 74 , 1801 – 1806 ( 1989. ). [PubMed] [Google Scholar]
  • 7. ) Medeiros , L. J. , van Krieken , J. H. , Jaffe , E. S. and Raffeld , M.Association of bcl‐1 rearrangements with lymphocytic lymphoma of intermediate differentiation . Blood , 76 , 2086 – 2090 ( 1990. ). [PubMed] [Google Scholar]
  • 8. ) Williams , M. E. , Meeker , T. C. and Swerdlow , S. H.Rearrangement of the chromosome 11 bcl‐l locus in centrocytic lymphoma: analysis with multiple breakpoint probes . Biood , 78 , 493 – 498 ( 1991. ). [PubMed] [Google Scholar]
  • 9. ) Berenson , J. R. , Yang , J. and Mickel , R. A.Frequent amplification of the bcl‐1 locus in head and neck squamous cell carcinomas . Oncogene , 4 , 1111 – 1116 ( 1989. ). [PubMed] [Google Scholar]
  • 10. ) Lammie , G. A. , Fantl , V. , Smith , R. , Schuuring , E. , Brookes , S. , Michalides , R. , Dickson , C. , Arnold , A. and Peters , G.D11S287, a putative oncogene on chromosome 11q13, is amplified and expressed in squamous cell and mammary carcinomas and linked to BCL‐1 . Oncogene , 6 , 439 – 444 ( 1991. ). [PubMed] [Google Scholar]
  • 11. ) Schuuring , E. , Verhoeven , E. , Mooi , W. J. and Michalides , R. J. M. A.Identification and cloning of two overexpressed genes, U21B31/PRAD1 and EMS1, within the amplified chromosome 11q13 region in human carcinomas . Oncogene , 7 , 355 – 361 ( 1992. ). [PubMed] [Google Scholar]
  • 12. ) Liscia , D. S. , Merlo , G. R. , Garrett , C. , French , D. , Mariani‐Constantiti , R. and Callahan , R.Expression of int‐2 mRNA in human tumors amplified at the int‐2 locus . Oncogene , 4 , 1219 – 1224 ( 1989. ). [PubMed] [Google Scholar]
  • 13. ) Tsuda , T. , Tahara , E. , Kajiyama , G. , Sakamoto , H. , Terada , M. and Sugimura , T.High incidence of coamplification of hst‐1 and int‐2 genes in human esophageal carcinomas . Cancer Res. , 49 , 5505 – 5508 ( 1989. ). [PubMed] [Google Scholar]
  • 14. ) Fantle , V. , Richards , M. A. , Smith , R. , Lammie , G. A. , Johnstone , G. , Allen , D. , Gregory , W. , Peters , G. , Dickson , C. and Barnes , D. M.Gene amplification on chromosome band 11q13 and oestrogen receptor status in breast cancer . Eur. J. Cancer , 26 , 423 – 429 ( 1990. ). [DOI] [PubMed] [Google Scholar]
  • 15. ) Arnold , A. , Kim , H. G. , Gaz , R. D. , Eddy , R. L. , Fukushima , Y. , Byers , M. G. , Shows , T. B. and Kronenberg , H. M.Molecular cloning and chromosomal mapping of DNA rearranged with the parathyroid hormone gene in a parathyroid adenoma . J. Clin. Invest. , 83 , 2034 – 2040 ( 1989. ). [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 16. ) Rosenberg , C. L. , Kim , H. G. , Shows , T. B. , Kronenberg , H. M. and Arnold , A.Rearrangement and overexpression of D11S287E, a candidate oncogene on chromosome 11q13 in benign parathyroid tumors . Oncogene , 6 , 449 – 453 ( 1991. ). [PubMed] [Google Scholar]
  • 17. ) Withers , D. A. , Harvey , R. C. , Faust , J. B. , Melnyk , O. , Carey , K. and Meeker , T. C.Characterization of a candidate bcl‐1 gene . Mol Cell, Biol , 11 , 4846 – 4853 ( 1991. ). [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 18. ) Rosenberg , C. L. , Wong , E. , Petty , E. M. , Bale , A. E. , Tsujimoto , Y. , Harris , N. L. and Arnold , A.PRAD1, a candidate BCL1 oncogene: mapping and expression in centrocytic lymphoma . Proc. Natl. Acad. Sci. USA , 88 , 9638 – 9642 ( 1991. ). [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 19. ) Seto , M. , Yamamoto , K. , Lida , S. , Akao , Y. , Utsumi , K. , Kubonishi , L , Miyoshi , L , Ohtsuki , T. , Yawata , Y. , Namba , M. , Motokura , T. , Arnold , A. , Takahashi , T , and Ueda , R.Gene rearrangement and overexpression of PRAD1 in lymphoid malignancy with t(11;14)(q13;q32) translocation . Oncogene , 7 , 1401 – 1406 ( 1992. ). [PubMed] [Google Scholar]
  • 20. ) Komatsu , H. , Yoshida , K. , Seto , M. , Iida , S. , Aikawa , T. , Ueda , R. and Mikuni , C.Overexpression of PRAD1 in a mantle zone lymphoma patient with a t(11;22)(q13;q11) translocation . Br. J. Haematol. , 85 , 427 – 429 ( 1993. ). [DOI] [PubMed] [Google Scholar]
  • 21. ) Motokura , T. , Bloom , T. , Kim , H. G. , Jüppner , H. , Ruderman , J. V. , Kronenberg , H. M. and Arnold , A.A novel cyclin encoded by a bcll‐linked candidate oncogene . Nature , 350 , 512 – 515 ( 1991. ). [DOI] [PubMed] [Google Scholar]
  • 22. ) Xiong , Y. , Connolly , T. , Futcher , B. and Beach , D.Human D‐type cyclin . Cell , 65 , 691 – 699 ( 1991. ). [DOI] [PubMed] [Google Scholar]
  • 23. ) Schuuring , E. , Verhoeven , E. , Litvinov , S. and Michalides , R. J. M. A.The product of the EMS1 gene, amplified and overexpressed in human carcinomas, is homologous to a v‐src substrate and is located in cell‐substratum contact sites . Mol. Cell. Biol , 13 , 2891 – 2898 ( 1993. ). [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 24. ) Akao , Y. , Seto , M. , Yamamoto , K. , Iida , S. , Nakazawa , S. , Inazawa , J. , Abe , T. , Takahashi , T. and Ueda , R.The RCK gene associated with t(11;14) translocation is distinct from the MLL/ALL‐1 gene with t(4;11) and t(11;19) translocations . Cancer Res. , 52 , 6083 – 6087 ( 1992. ). [PubMed] [Google Scholar]
  • 25. ) Seto , M. , Takahashi , T. , Tanimoto , M. and Nishizuka , Y.Production of monoclonal antibodies against MM antigen: the serologic identification of MM antigen with Ly‐6.2 alloantigen . J. Immunol. , 128 , 201 – 205 ( 1982. ). [PubMed] [Google Scholar]
  • 26. ) Daibata , M. , Takasaki , M. , Hirose , S. , Kubonishi , I. , Taguchi , H. , Ohtsuki , Y. and Miyoshi , I.Establishment of a new human B cell line carrying t(11;14) chromosome abnormality . Jpn. J. Cancer Res. , 78 , 1182 – 1185 ( 1987. ). [PubMed] [Google Scholar]
  • 27. ) Ohtsuki , T. , Yawata , Y. , Wada , H. , Sugihara , T. , Mori , M. and Namba , M.Two human myeloma cell lines, amylase‐producing KMS‐12‐PE and amylase‐non‐producing KMS‐12‐BM, were established from a patient, having the same chromosome marker, t(11;14)(q13;q32) . Br. J. Haematol. , 73 , 199 – 204 ( 1989. ). [DOI] [PubMed] [Google Scholar]
  • 28. ) Hirano , T. , Hirohashi , S. , Kunii , T. , Noguchi , M. , Shimosato , Y. and Hayata , Y.Quantitative distribution of cluster 1 small cell lung cancer antigen in cancerous and non‐cancerous tissues, cultured cells and sera . Jpn. J. Cancer Res. , 80 , 348 – 355 ( 1989. ). [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 29. ) DeLellis , R. A. , Sternberger , L. A. , Mann , R. B. , Banks , P. M. and Nakane , P. K.Immunoperoxidase technics in diagnostic pathology. Report of a workshop sponsored by the National Cancer Institute . Am. J. Clin. Pathol , 71 , 483 – 488 ( 1979. ). [DOI] [PubMed] [Google Scholar]
  • 30. ) Shi , S. R. , Key , M. E. and Karla , K. L.Antigen retrieval in formalin‐fixed, paraffin‐embedded tissues: an enhancement method for immunohistochemical staining based on microwave oven heating of tissue sections . J. Histochem. Cytochem. , 39 , 741 – 748 ( 1991. ). [DOI] [PubMed] [Google Scholar]
  • 31. ) Baldin , V. , Lukas , J. , Marcote , M. J. , Pagano , M. and Draetta , G.Cyclin D1 is a nuclear protein required for cell cycle progression in G1 . Genes Dev. , 7 , 812 – 821 ( 1993. ). [DOI] [PubMed] [Google Scholar]
  • 32. ) Jiang , W. , Zhang , Y.‐J. , Kahn , S. M. , Hollstein , M. C. , Santella , R. M. , Lu , S.‐H. , Harris , C. C. , Montesano , R. and Weinstein , I. B.Altered expression of the cyclin D1 and retinoblastoma genes in human esophageal cancer . Proc. Natl. Acad. Sci. USA , 90 , 9026 – 9030 ( 1993. ). [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 33. ) Inaba , T. , Matsushime , H. , Valentine , M. , Roussel , M. F. , Sherr , C. J. and Look , A. T.Genomic organization, chromosomal localization, and independent expression of human cyclin D genes . Genomics , 13 , 565 – 574 ( 1992. ). [DOI] [PubMed] [Google Scholar]
  • 34. ) Xiong , Y. , Zhang , H. and Beach , D.D type cyclins associates with multiple protein kinases and the DNA replication and repair factor PCNA . Cell , 71 , 505 – 514 ( 1992. ). [DOI] [PubMed] [Google Scholar]
  • 35. ) Seto , M. , Jaeger , U. , Hockett , R. D. , Graninger , W. , Bennett , S. , Goldman , P. and Korsmeyer , S. J.Alternative promoters and exons, somatic mutation and deregulation of the Bcl‐2‐Ig fusion gene in lymphoma . EMBO J. , 7 , 123 – 131 ( 1988. ). [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Japanese Journal of Cancer Research : Gann are provided here courtesy of Wiley

RESOURCES