Fig 7. MPA, unlike NET, increases CD4 and CCR5 mRNA levels in TZM-bls, via a GR-dependent mechanism.

TZM-bl cells were stimulated for 24 hours with indicated ligands (a-b) 100 nM MPA, 100 nM NET or vehicle control (EtOH) (0.1% v/v ethanol) or (c-d) 10 nM MPA or (e-f) 100 nM MPA, 100 nM RU486 (RU), or combinations thereof (RU/MPA). RNA was isolated, cDNA was synthesised and relative CCR5, CD4 mRNA levels were determined by real time qPCR, normalized to GAPDH and relative fold change in expression was determined by setting vehicle control to 1. Results are shown from 6 independent experiments for each panel and is represented as mean ± SEM. Statistical significance was determined by using (a-b, e-f) one-way ANOVA with a post hoc Tukey tests between conditions or (c-d) using a parametric unpaired t-test comparing the vehicle to 10 nM MPA with * or ** denoting p<0.05 or p<0.01 respectively.