Fig 4. Surfactin, iturin A, and fengycin inhibited the growth and formation of spiral hyphae of S. scabies PS07.
A. Disk diffusion assays were used to test the anti-S. scabies activity of surfactin, iturin A, and fengycin. Approximately 106 S. scabies spores were spread on YME solid agar, and a 6-mm disk containing iturin A (dissolved in ethanol), surfactin (dissolved in methanol), or fengycin (dissolved in methanol) were pressed on the surface of an agar plate and incubated at 28°C for five days. B. The morphology of S. scabies PS07 in the undifferentiating zone of panel A was observed under a scanning electron microscope. (a) The magnification is 5,000X, and the scale bar represents 10 μm. (b) The magnification is 15,000X, and the scale bar represents 2.5 μm.