Fig 2. SIVcpz is restricted to CPZ CCR5, while SIVmus1085 can use MUS and GSN CXCR6 and CCR5 for entry.

A) Left panel: CF2thLuc cells that contain a Tat-driven luciferase reporter were transfected with expression plasmids containing chimpanzee coreceptor and CD4 or empty vector (No CoR). 48 hours later cells were infected with one of four diverse SIVcpz isolates derived from infectious molecular clones. Entry was quantified 48 hours later by lysing cells and measuring luciferase content by relative light units (RLU). Right panel: 293T cells were transfected with cpzCD4 and coreceptor and infected with luciferase reporter pseudotypes expressing a promiscuous SIVsmm Env that can use a wide repertoire of coreceptors for entry. B-C) 293T cells were transfected with expression plasmids containing coreceptor or empty vector (No CoR), in conjunction with CD4, of MUS (B) or GSN (C) origin. 48 hours later cells were infected with luciferase reporter viruses carrying SIVmus1085 Envs (left panel) or a promiscuous SIVsmm (right panel). Entry was quantified 72 hours later by lysing cells and measuring luciferase content by relative light units (RLU). Infections were carried out in triplicate and data represent means ± one standard deviation.