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. 2018 Apr 16;14(4):e1007003. doi: 10.1371/journal.ppat.1007003

Fig 5. SIVmus1085 Env determinants of CXCR6 usage.

Fig 5

A) SIVmus1085 1–54 and SIVcpz MT145 env constructs were mutated to generate V3 loop mutants SIVmusA326P and SIVcpzP326A. Luciferase reporter pseudotyped viruses carrying these Envs, or SIVmus 1085 1–54 or SIVcpz MT145, were used to infect 293T cells expressing musCD4 and coreceptor (M) or cpzCD4 and coreceptor (C). Entry was quantified 72 hours post-infection by relative light units (RLU). B) The V3 loop of SIVcpz MT145 was replaced with the V3 loop of SIVmus1085 1–54 to generate SIVcpz-musV3. Pseudotypes carrying the SIVcpz-musV3 Env or SIVcpz MT145, were used to infect 293T cells expressing musCD4 and coreceptor (M) or cpzCD4 and coreceptor (C). Due to low infectivity of SIVcpz-musV3, entry was normalized to % entry through musCD4 and musCCR5 to enable comparison. Infections were carried out in triplicate and data represent means ± one standard deviation.