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. 2018 Apr 26;13(4):e0196409. doi: 10.1371/journal.pone.0196409

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© 2018 Manjunatha et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 2 — Agarose gel showing specificity (A) and sensitivity (B) using PtRA₆₈F/R primers set. (A) Specificity test using DNA templates of different Puccinia spp. Lanes: M: 1 kb ladder, Fermentas; 1–10: DNA template of P. triticina (1–12, 2-12-1, 3-12-2, 4–77, 5-77-1, 6-77-2,7-77-5, 8-77-6, 9-77-8, 10-104-2); 11–13: P. striiformis f. sp. tritici (46S119, 38S102 and 78S84); 14–15: P. graminis f. sp. tritici (40–1 and 40-A); 16-Bipolaris sorokiniana (BS-75); 17: B. oryzae (BO-1); 18:-Healthy wheat leaf DNA; 19: sterile water. (B) Sensitivity test using different concentration of template DNA. Lanes: M: 1kb ladder, Fermentas; 1: 100ng; 2: 50ng; 3: 10ng; 4: 1ng; 5: 500pg; 6: 100pg; 7: 50pg; 8: 25pg DNA as a template, 9: sterile water.