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. 2018 Apr 26;13(4):e0196409. doi: 10.1371/journal.pone.0196409

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© 2018 Manjunatha et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 4 — (A) Standard curve showing the log₁₀ DNA amount (fg) plotted against cycle threshold (Ct) for different dilutions of genomic DNA of P. triticina (pathotype 77–5) in PCR grade water. (B) Melting curve obtained through real time PCR products using P. triticina specific primers, no peak was observed in negative control.