Table 2. Details of the LAMP primers used in the development of diagnostic assay.
Name | Sequence | Length (bp) |
---|---|---|
A | Conventional PCR Primers | |
PtRA68F | CTACTCAGCCAGAAGCACCTC | 21 |
PtRA68R | TACTGACCACCAGCGTCTTG | 20 |
B | Real time PCR Primers | |
PtRA68(RT)F | CTGAGTCTGCTTGTAGTTGGG | 21 |
PtRA68(RT)R | CTTGCTTTTCGGGCTTGATG | 20 |
C | LAMP Primers | |
F3 | TCGACAGACTGAGTCTGCT | 19 |
B3 | CGATCTTGATGCGCATCTCC | 20 |
FIP | TGTGCTGGATGGCGGATAGGAT-GTTGGGGTCATTCTCAACCG | 42 |
BIP | CATCGGGACATCAAGCCCGAA-TCAACACAAAGTCAGCCGT | 40 |
LF | GGCATAGAACCTGGCACAGT | 20 |
LB | AAGCAAGCCTCCATTCTCACA | 21 |