Fig 6. Transcriptional activity/functional WUS is required for stabilizing the WUS protein.

The pWUS::eGFP-WUS (mHOD1+ΔHOD2) accumulation in wild type SAMs upon Mock (A), 6 hrs (B), and 24 hrs (C) of 6-BAP treatments. Note the diffuse accumulation of WUS and the response to 6-BAP treatments, particularly in the pith cells in (B-C). The pWUS::eGFP-WUS (mHOD1+ΔHOD2) accumulation in wus-1 SAMs upon Mock (D), 6 hrs (E) and 24 hrs (F) of 6-BAP treatments. Note the poor accumulation of WUS in (D) and the much weaker response to 6-BAP treatments in (E-F). Cytokinin signaling response from the pTCS::mGFP5-ER in wus-1 SAMs after Mock (G), 6 hrs (H), and 24 hrs (I) of 6-BAP treatments. The cytokinin response is clearly evident even after 6 hrs of treatment compared to Mock, demonstrating that cytokinin signaling is normal in wus-1 SAMs and that the transcriptional activity/function of WUS is required for WUS stabilization. (A-F) are expressed from pWUS. The cell layers in SAMs are marked; the L1 and the L2 are monolayers. The multilayer L3 has been divided into the apical L3 layer and the basal L3 layers. The pith is located beneath the basal L3 layers. Insets for each image show the areas identified by black arrowheads at 4x zoom and white arrowheads show boundaries of the reporter accumulation. Autofluorescence is denoted by grey arrowheads and is characterized by multiple foci in a single cell. eGFP (green) is overlaid on FM4-64 (red) plasma membrane stain. The scale bar is 50 μm for all images.