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. 2018 Apr 16;14(4):e1007346. doi: 10.1371/journal.pgen.1007346

Fig 5. Functional validation of SOX9 and GLI cooperation.

Fig 5

(A) Relative expression levels of SOX9-GLI common target genes in Sox9+/+ and Sox9+/- limb chondrocytes (E13.5). (B) Relative expression levels of SOX9-GLI common target genes in Gli2+/+ and Gli2-/- limb chondrocytes (E13.5). Gapdh was used as the endogenous control. The error bar represents the standard deviation of the relative expression level of each gene in independent triplicate. (C) SOX9 activated the expression of Ptch1 promoter in a dosage dependent manner in transiently transfected ATDC5 cells. (D-H) SOX9 increased GLI-dependent activation of Ptch1, Gli1, Gli2, Sox9 and Col2a1 regulatory elements in transiently transfected ATDC5 cells. Expression plasmids for Sox9, Gli1, Gli2 and their combination were cotransfected as indicated below the bars. Equal amounts of expression plasmids were used for each well. **: p-value < 0.01; *: p-value < 0.05; NS: Non-significant.