Table 1. High-confidence RNAi screen results for control cells.

FlyBase ID	Gene Symbol	Human Orthologa	Treatment	Directionb	dsRNAsc	Avg Z-Score
FBgn0039830	ATPsynC	ATP5G2	Control	Down	2	−2.17
FBgn0026872	CG14777	MPV17L	Control	Down	2	−1.82
FBgn0051663	CG31663	–	Control	Down	2	−1.58
FBgn0052512	CG32512	TMEM205	Control	Down	2	−2.49
FBgn0039223	CG5805	SLC25A44	Control	Down	2	−1.97
FBgn0032833	COX4	COX4I1,2	Control	Down	2	−1.76
FBgn0040529	COX7A	COX7A2	Control	Down	2	−2.02
FBgn0062413	Ctr1A	SLC31A1	Control	Down	2	−2.06
FBgn0262743	Fs(2)Ket	KPNB1	Control	Down	2	−3.91
FBgn0024319	Nach	SCNN1B,G	Control	Down	2	−1.89
FBgn0035382	Or63a	–	Control	Down	2	−1.73
FBgn0021906	RFeSP	UQCRFS1	Control	Down	2	−2.93
FBgn0266098	rg	NBEA	Control	Down	2	−1.82
FBgn0003360	sesB	SLC25A4	Control	Down	2	−1.83
FBgn0025725	αCOP	COPA	Control	Up	2	2.11
FBgn0038415	CG17929	–	Control	Up	2	2.17
FBgn0264907	CG44098	–	Control	Up	2	1.76
FBgn0027556	CG4928	UNC93A	Control	Up	2	1.99
FBgn0036742	CG7497	PTGER1,3,4	Control	Up	2	1.71
FBgn0034438	CG9416	ERMP1	Control	Up	2	1.85
FBgn0043903	dome	–	Control	Up	2	1.82
FBgn0000636	Fas3	–	Control	Up	2	2.97
FBgn0001179	hay	ERCC3	Control	Up	2	3.19
FBgn0263782	Hmgcr	HMGCR	Control	Up	2	2.59
FBgn0051072	Lerp	IGF2R	Control	Up	2	1.69
FBgn0259214	PMCA	ATP2B1,2,3,4	Control	Up	2	2.29
FBgn0032006	Pvr	FLT1	Control	Up	2	5.12
FBgn0086357	Sec61α	SEC61A1,2	Control	Up	2	2.48
FBgn0011708	Syx5	STX5	Control	Up	3	2.87
FBgn0021796	Tor	MTOR	Control	Up	2	2.51

ID, identifier; dsRNAs, double-stranded RNAs; Avg, average.

^aBest match ortholog(s) are shown (DIOPT score cutoff > 2) (Hu et al. 2011).

^bDown, decreased total ATP levels following plate-based normalization within a treatment group; up, increased total ATP levels following plate-based normalization within a treatment group.

^cNumber of unique dsRNAs in the library that target the gene. For all high-confidence hits as shown here, each of the designs resulted in a Z-score > 1.5 or < −1.5 and in the same direction as what was found for other designs targeting the same gene.