Figure 2.

Factors identified in the screens include histone modifiers and readers, and their regulators. (A) Bulk progeny of crosses combining the indicated boundary elements and ORF deletions, replicated onto medium lacking adenine [NBA (yeast nitrogen base) supplemented with leucine] to assay (EcoRV)::ade6⁺ expression. Light colors reflect (EcoRV)::ade6⁺ expression. Control: an ORF deletion with no effect on (EcoRV)::ade6⁺ expression (V3-P25-45, SPAC15E1.07c). (B) Schematic representation of factors and complexes shown in (A). HDACs remove acetyl groups (in green); CLRC methylates H3K9 (in red); CRL4^Cdt2, positively regulated by Csn1, destabilizes the antisilencing factor Epe1. HDACs, histone deacetylases; ORF, open reading frame.