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. 2017 Nov 29;8(2):477–489. doi: 10.1534/g3.117.300341

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Copyright © 2018 Jahn et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Effects of selected factors on (EcoRV)::ade6⁺ transcript in the presence of the WT boundary IR-R⁺, assayed by RT-QPCR. The strains are the same as in Figure 4. The means of three biological replicates are displayed. (EcoRV)::ade6⁺ RNA level was normalized to act1⁺ in each strain and is expressed as % expression in IR-RΔ cells. The y-axis labeling on the left applies to the three strains on that side that have the highest (EcoRV)::ade6⁺ expression. The labeling on the right applies to the other strains. Errors bars represent the SE of the mean. RT-QPCR, reverse transcription-quantitative polymerase chain reaction; WT, wild-type.