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. 2017 Nov 29;8(2):477–489. doi: 10.1534/g3.117.300341

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Copyright © 2018 Jahn et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Effects of Mcl1 and Wpl1 on heterochromatic silencing. (A) The mating-type regions used in this experiment featured two ectopic boundaries, STAR2 and the 85 bp STAR2-S element (Toteva et al. 2017), in addition to wild-type IR-R⁺. (B) mcl1Δ and wpl1Δ mutants with the indicated boundaries were spotted on low adenine to compare (EcoRV)::ade6⁺ expression with the wild-type background. mcl1Δ confers a pronounced growth defect; however, the formed (EcoRV)::ade6⁺ mcl1Δ colonies are Ade⁺. wpl1Δ induces strong variegation. The strains were wild-type: PG3947, PG3950, PC152, and PT600; mcl1Δ: PA71, PA72, PA73, and PA74; and wpl1Δ: PA82, PA83, PA84, and PA85. YE, medium with limited adenine.