Table 1. List of silencing factors identified in this study, with references to prior identification in genetic screens or candidate and biochemical approaches.
| Gene ID | Product | Description | Reference |
|---|---|---|---|
| Histone deacetylase complexes | |||
| SPBC2D10.17 | Clr1 | SHREC complex cryptic loci regulator 1 subunit | Thon and Klar (1992), Sugiyama et al. (2007) |
| SPAC1B3.17 | Clr2 | SHREC complex cryptic loci regulator 2 subunit | Ekwall and Ruusala (1994), Thon et al. (1994), Sugiyama et al. (2007) |
| SPBC800.03 | Clr3 | SHREC complex cryptic loci regulator 3 subunit (histone deacetylase) | Ekwall and Ruusala (1994), Thon et al. (1994), Sugiyama et al. (2007) |
| SPBP35G2.10 | Mit1 | SHREC complex ATP-dependent DNA helicase subunit Mit1 | Sugiyama et al. (2007) |
| SPAC16C9.05 | Cph1 | Clr6 histone deacetylase-associated PHD protein-1 | Nicolas et al. (2007) |
| Histone H3K9 methyltransferase complex CLRC and Dos2 complexes | |||
| SPBC428.08c | Clr4 | Cryptic loci regulator 4 (histone H3K9 methyltransferase) | Ekwall and Ruusala (1994), Thon et al. (1994), Hong et al. (2005), Horn et al. (2005) |
| SPCC970.07c | Raf2/Dos2/Cmc2/Clr7 | Rik1-associated factor 2/delocalization of Swi6 factor 2/cullin-associated methyltransferase complex protein 2/cryptic loci regulator 7 | Hong et al. (2005), Horn et al. (2005), Li et al. (2005), Thon et al. (2005) |
| SPCC613.12c | Raf1/Dos1/Cmc1/Clr8 | Rik1-associated factor 1/delocalization of Swi6 factor 2/cullin-associated methyltransferase complex protein 1/cryptic loci regulator 8 | Hong et al. (2005), Horn et al. 2005), Li et al. (2005), Thon et al. (2005) |
| SPCC11E10.08 | Rik1 | Silencing protein Rik1 | Ekwall and Ruusala (1994), Hong et al. (2005), Horn et al. (2005), Thon et al. (2005) |
| SPAC1071.02 | Mms19 | Dos2 silencing complex subunit | Li et al. (2011) |
| HP1 family and associated factors | |||
| SPAC664.01c | Swi6 | Chromodomain protein Swi6 | Gutz and Schmidt (1985) |
| SPAC1851.03 | Ckb1 | CK2 family regulatory subunit | Shimada et al. (2009), Braun et al. (2011) |
| CRL4 | |||
| SPAC17H9.19c | Cdt2 | WD repeat protein Cdt2 | Braun et al. (2011), Bayne et al. (2014) |
| SPAC17H9.10c | Ddb1 | Damaged DNA binding protein 1 | Braun et al. (2011), Bayne et al. (2014) |
| CSN | |||
| SPBC215.03c | Csn1 | COP9 signalosome complex subunit Csn1 | Bayne et al. (2014) |
| RPC | |||
| SPAC694.06c | Mrc1 | Mediator of replication checkpoint 1 | |
| SPAPB1E7.02c | Mcl1 | DNA polymerase α accessory factor | Mamnun et al. (2006), Natsume et al. (2008) |
| SPBC216.06c | Swi1 | Replication fork protection complex subunit Swi1 | |
| SPBC30D10.04 | Swi3 | Replication fork protection complex subunit Swi3 | |
| SPBC609.05 | Pob3 | FACT complex subunit | Lejeune et al. (2007) |
| Ubiquitin–SUMO-related | |||
| SPCC338.16 | Pof3 | F-box protein | Katayama et al. (2002), Mamnun et al. (2006) |
| SPBC354.10 | Def1 | RNAPII degradation factor (predicted) | |
| SPAC1687.05 | Pli1 | SUMO E3 ligase | Xhemalce et al. (2004) |
| Genome organization/replication timing | |||
| SPBC16G5.15c | Fkh2 | Forkhead transcription factor Fkh2 | |
| SPBC428.17c | Wpl1 | Cohesin loading/unloading factor (WAPL) Wpl1 | |
| SPAC6F6.17 | Rif1 | Telomere length regulator protein Rif1 | Toteva et al. (2017) |
| SPBC1198.11C | Reb1 | RNA polymerase I transcription termination factor /replication block | Jakociunas et al. (2013) |
| Chromatin remodelers/chaperones | |||
| SPAC25A8.01c | Fft3 | Fission yeast fun 30 related protein Fft3 ATP-dependent helicase, chromatin remodeler | Stralfors et al. (2011) |
| SPAC31G5.19 | Abo1 | ATPase with bromodomain protein | Gal et al. (2016) |
| SPBC1347.02 | Fkpb39 | FKBP-type peptidyl-prolyl cis-trans isomerase (predicted) | |
| SPAC57A7.12 | Ssz1 | Heat shock protein | |
| Histone acetyltransferase | |||
| SPAC1952.05 | Gcn5 | SAGA complex histone acetyltransferase catalytic subunit Gcn5 | |
| RNA-associated proteins | |||
| SPAC4F10.14c | Btf3 | Nascent polypeptide-associated complex β subunit/basic transcription factor Btf3 | |
| SPCC4G3.15c | Not2 | CCR4-NOT complex NOT box subunit Not2 | Cotobal et al. (2015) |
| SPAC29B12.06c | Rcd1 | CCR4-NOT complex RNA-binding protein subunit Rcd1 | Cotobal et al. (2015) |
| Miscellaneous | |||
| SPBC428.07 | Meu6 | Meiotic chromosome segregation protein Meu6 (adjacent to clr4+ gene) | |
ID, identifier; PHD, Plant homeodomain; CLRC, Clr4 complex; FACT, facilitates chromatin transcription; RNAPII, RNA polymerase II.