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. 2018 Apr 3;128(5):1852–1866. doi: 10.1172/JCI89867

Figure 2. Phenotype of TTP–/– S100A9–/– mice.

Figure 2

(A) Disease progression of TTP–/– S100A9–/– mice versus controls (10 mice per group) is shown by the adapted PASI scores from P1–P9. *P < 0.05; **P < 0.01; ***P < 0.001, Mann-Whitney U test. (B) Photographs and skin sections of TTP–/– S100A9–/–, TTP–/–, S100A9–/–, and WT mice at day 9 after birth. Sections were stained with H&E or by immunohistochemistry for S100A8 or immunofluorescence for the indicated epidermal differentiation markers or invading T cells (CD3), granulocytes (Gr1), and macrophages (F4/80) (fluorescence, green signal) and Ki67 (fluorescence, red signal). Blue and red counterstaining shows nuclei. Scale bars: 200 μm (H&E); 50 μm (immunostaining). (C) Epidermis thickness was determined for at least 3 sections per mouse and for each section at 3 different sites and at least 5 mice for each group. (D and E) Expression of IFN-γ, IL-6, IL-17, IL-22, IL-23, and IL-36 in the skin of TTP–/– S100A9–/– mice at day 9 after birth compared with TTP–/– and S100A9–/– mice. The mRNA levels in the skin were measured by qRT-PCR (D) and quantified at the protein level by FACS-based analysis (E) of 3 independent experiments. Data represent mean ± SEM. *P < 0.05; **P < 0.01; ***P < 0.001, 1-way ANOVA (C–E).