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. 2018 Apr 3;128(5):1888–1902. doi: 10.1172/JCI97739

Figure 4. Methyldopa blocks DQ8 antigen presentation in vivo.

Figure 4

(A) Diagram of the assay used to monitor the potency of methyldopa to block DQ8 in Tg mice. (B) Adult DQ8-Tg mice were gavaged with vehicle or 200 mg/kg methyldopa 3 times per day for 4 days (n = 4/group). Ex vivo splenocytes were used as APCs to present different concentrations of the insulin B:13-23 peptide or a deamidated α-gliadin peptide to clone 5 or 489, respectively. No methyldopa was added to the in vitro culture. A dose of 200 mg/kg is equivalent to 1,000 mg 3 times per day in a human weighing 60 kg. Data represent the mean ± SEM and are representative of 3 independent experiments. (C) Percentages and (D) numbers of CD11b+ and CD11c+ cells in the spleens of treated mice. (E) MFI of DQ8 cell-surface staining on each cell population. Each dot represents an individual mouse.