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. 2018 Apr 9;128(5):1937–1955. doi: 10.1172/JCI95089

Figure 4. Loss of ZMYND8 suppresses breast tumor growth and metastasis to the lungs in mice.

Figure 4

(A–C) Growth of parental, ZMYND8- KO1 (A) and -KO2 (B) MDA-MB-231, and ZMYND8-KO1 MCF-7 (C) tumors in mice (mean ± SEM, n = 4). ****P < 0.0001 versus parental by 2-way ANOVA with Sidak’s t test. The image of parental and ZMYND8-KO1 tumors harvested at the end time point is shown in the insets. ZMYND8-KO in tumors was confirmed by immunoblot assays (bottom). (D–F) Representative H&E and immunohistochemical staining of CC3 and endomucin in parental or ZMYND8-KO1 MDA-MB-231 tumors (D). Magnified images of the boxed area are shown in the insets. Scale bar, 200 μm. CC3-positive cell numbers (E) and endomucin-positive areas (F) in tumors are quantified (mean ± SEM, n = 4). **P < 0.01, ****P < 0.0001 versus parental by 2-tailed Student’s t test. (G–J) Lung metastasis in mice bearing parental or ZMYND8-KO1 or -KO2 MDA-MB-231 tumors by H&E staining (G and I) and qPCR assays (H and J, mean ± SEM, n = 4). Magnified images of the boxed area are shown in the insets (G and I). *P < 0.05, **P < 0.01 versus parental by 2-tailed Student’s t test. Scale bar, 200 μm. (K) Lung colonization of parental and ZMYND8-KO MDA-MB-231 cells by qPCR assays (mean ± SEM, n = 11). ****P < 0.0001 versus parental by 1-way ANOVA with Dunnett’s test. (L) Circulating tumor cells in blood from mice bearing parental or ZMYND8-KO1 MDA-MB-231 tumors by qPCR assays (mean ± SEM, n = 4). ***P < 0.001 versus parental by 2-tailed Student’s t test.