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. 2018 Apr 9;128(5):1937–1955. doi: 10.1172/JCI95089

Figure 5. HIF-1 and HIF-2 are required for ZMYND8-mediated breast tumor growth and metastasis in mice.

Figure 5

(A) Growth of Parental+EV, Parental+ZMYND8, HIF-1/2α–DKO+EV, and HIF-1/2α–DKO+ZMYND8 MDA-MB-231 tumors in mice (mean ± SEM, n = 5). **P < 0.01, ****P < 0.0001 by 2-way ANOVA with Tukey’s t test. NS, not significant. (B–D) Representative H&E and immunohistochemical staining of CC3 and endomucin in primary tumors. Magnified images of the boxed area are shown in the insets. Scale bar, 200 μm. CC3-positive cell numbers (C) and endomucin-positive areas (D) in tumors are quantified (mean ± SEM, n = 5). *P < 0.05, ****P < 0.0001 by 1-way ANOVA with Sidak’s t test. (E) Lung metastasis are quantified by qPCR (mean ± SEM, n = 5). *P < 0.05, ****P < 0.0001 by 1-way ANOVA with Dunnett’s test.