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(A) Co-IP assays of endogenous ZMYND8 and HIF-1α or HIF-2α in MDA-MB-231 cells exposed to 1% O2 for 6 hours (n = 3). WCL, whole-cell lysate. (B and C) Mapping of the ZMYND8 domain binding to HIF-1α. Schematic depiction of FL and domain-deleted ZMYND8 (B). Co-IP assays of endogenous HIF-1α and FL or truncated FLAG-ZMYND8 in transfected HEK293T cells exposed to 1% O2 for 6 hours (n = 3) (C).
