Figure 2. Effects of intraperitoneally administered anti–apoE antibodies on amyloid pathology in APPPS1-21/APOE4 mice.

At the age of 2 months, the mice were injected i.p. weekly with 50 mg/kg antibodies (all female, n = 10–13/group). The mice were sacrificed at the age of 3.5 months and the Aβ pathology in the brain was assessed using histology and biochemical approaches. (A) Representative Aβ staining using HJ3.4 (left panel) and representative X-34 staining for fibrillar plaques (right panel). Scale bar = 1 mm. (B) Quantification of HJ3.4 staining. (C) Quantification of X-34 staining. (D) Aβ₄₀ and (E) Aβ₄₂ was measured by ELISA in insoluble fractions of tissue lysates (Guan = Guanidine) by ELISA. One-way ANOVA followed by Tukey’s t test was performed to compare different groups shown in B–E. Data are mean ± SEM. *P < 0.05, **P < 0.01.