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. 2018 Apr 3;128(5):1820–1836. doi: 10.1172/JCI97280

Figure 2. Conditional deletion of Toso in B cells results in increased mortality and reduced T cell cytokine responses upon influenza infection.

Figure 2

(A–G) Mice were infected i.n. with 1,000 PFU influenza virus strain A/PR8 (H1N1). (A–C) Survival of mice was monitored over time. (A) CD4-Cre+/– and Tosof/f/CD4-Cre+/– mice (n = 9). (B) CD11c-Cre+/– and Tosof/f/CD11c-Cre+/– mice (n ≥14). (C) CD19-Cre+/– mice and Tosof/f/CD19-Cre+/– mice (n ≥8). **P < 0.005; log-rank test. (D–G) Lung cells isolated on day 9 p.i. were restimulated ex vivo, and the number and frequency of IFN-γ–producing (D and E) and TNF-α–producing (F and G) CD4+ T cells (D and F) and CD8+ T cells (E and G) were quantified by intracellular cytokine staining. (H and I) CD19-Cre+/– mice and Tosof/f/CD19-Cre+/– mice were infected i.n. with 50 PFU influenza virus strain A/PR8 (H1N1). On day 7 p.i., spleens were analyzed for frequency and number of PD-1–positive CD4+ T cells (H) and PD-L2–positive CD19+ B cells (I). (D–I) Each symbol represents an individual mouse; horizontal lines indicate the mean (± SEM). (D–G) n = 5; (H and I) n = 4. *P < 0.05; **P < 0.01; ***P < 0.001; Student’s t test. Data are representative of at least 3 independent experiments.