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Distance bars represent 275 μM. (A) HE, PARP-1, and [125I]KX-1 autoradiograph on adjacent tissue sections showed colocalization between [125I]KX1 and PARP-1 c-IHC. (B) In vitro autoradiography showed a difference in PARP-1 expression that was also confirmed by PARP-1 f-IHC. Max intensity by autoradiograph was 0.28 vs. 0.20 μCi/mg and fluorescent intensity of PARP-1 from whole-section f-IHC was 9.6 vs. 6.7 RFU.
