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. 2018 Mar 26;128(5):1807–1819. doi: 10.1172/JCI99088

Figure 2. PAI1 affects fibrogenesis during the inflammation phase in Snail-Tg skin.

Figure 2

(A) WT, Snail-Tg, and Snail-Tg/Pai1-KO pup skin sections were analyzed for TENC localization by immunostaining (n = 3; scale bar and original magnification: 50 μm) and total TENC protein levels by Western blotting (n = 3). (B) Quantification of secreted TENC in recombinant PAI1-treated fibroblast conditioned media (n = 3). qPCR for proinflammatory cytokines Tnfa, Il4, and Ifng in (C) neonatal WT, Snail-Tg, and Snail-Tg/Pai1-KO skin sections (n = 3) and (D) recombinant PAI1–treated fibroblasts (n = 3). (E and F) Neonatal WT, Snail-Tg, and Snail-Tg/Pai1-KO pup skin sections were analyzed for (E) the cytokines Cxcl1, Cxcl5, Cxcl9, Cxcl10, and Cxcl11 by qPCR (n = 3) and (F) macrophages by immunostaining (left; scale bar: 50 μm; n = 3) and mast cells by toluidine blue staining (right; scale bar: 10 μm; n = 4). (G) Quantification of cell numbers in F. Data represent the mean ± SEM. *P < 0.05 and **P < 0.01, by Student’s t test (B and D) and 1-way ANOVA followed by Tukey’s post hoc analysis (A, C, E, and G).