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. 2018 Apr 27;8:94. doi: 10.1038/s41398-018-0142-6

Fig. 4. Loss of Shank3 in selected striatal neurons leads to cell autonomous alterations of synaptic function and PSD components.

Fig. 4

(a) Representative traces of evoked action potentials in D1 MSNs neurons from Drd1-Shank3 WT (+/+) (black) and KO (−/−) (red) mice. The action potentials reflect responses to 200, 300, and 400 pA current injections, respectively. b Summarized data for the number of evoked action potentials (APs) at the indicated amplitudes of current injection in D1 MSNs from Drd1-Shank3 WT (+/+) and KO (−/−) mice (2-way ANOVA, main effects of genotype and stimulation, p < 0.001, genotype x stimulation interaction, p < 0.001). c Example traces of evoked action potentials in D2 MSNs neurons from Drd2-Shank3 WT (+/+) (gray) and KO (−/−) (pink) mice. d Summarized data for the numbers of evoked action potentials at the indicated amplitudes of current injection in D2 MSNs from Drd2-Shank3 WT (+/+) and KO (−/−) mice (2-way ANOVA, main effects of genotype and stimulation, p < 0.001, genotype x stimulation interaction, p < 0.001). e−g Homer1b/c levels in the PSD from striatum where loss of Shank3 was targeted. (e) Dlx5/6-Shank3 mice show a reduction in Homer1b/c protein in striatal (ST) (p = 0.002), but not in cortical (CX) or hippocampal (HP) PSD samples; n = 5 mice/genotype. f Drd1-Shank3 mice have decreased Homer 1b/c in ST (p = 0.018), but not in the CX or HP samples; n = 4 mice/genotype. g Drd2-Shank3 mice have a loss of Homer1b/c in the ST (p < 0.001), but not in the CX or HP; n = 4 mice/genotype. For all westerns, independent samples two-tailed t-tests; representative images are shown and each western was replicated at least two times. For all panels, *p < 0.05, compared to wild-type controls. All data are expressed as means ± SEM