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. 2018 Apr 26;8:6553. doi: 10.1038/s41598-018-24516-7

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Experimental method. (a) Schematic illustration of the FUS experiment setup for the treatment of U87 tumor-bearing mice. (b) Bioluminescence image of the orthotopic mouse model with the green fluorescence image of the mouse brain shown on the right. (c) Schematic illustration of the MRgFUS system for the treatment of GL261 tumor-bearing mice. (d) Representative contrast-enhanced MR images acquired before and after FUS treatment. The enhanced accumulation of the MR contrast agents in the tumor region verified accurate tumor targeting by the FUS. (e) Diagram of FUS pulses. (f) Illustration of the experimental timeline.