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. 2018 Apr 26;8:6553. doi: 10.1038/s41598-018-24516-7

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Comparison of the circulating eGFP mRNA expression in the control and treated GL261 mice. Comparison of the expression levels ( $2^{{- ∆ C}_{T}}$ ) of (a) eGFP A and (b) eGFP B in the control group and three treatment groups with different acoustic pressures: 1.52 MPa, 2.74 MPa, and 3.53 MPa. All the measured data points as well as their mean and standard deviation are shown for each group. The circulating mRNA levels of eGFP A and eGFP B were significantly higher in the FUS-treated groups compared with the untreated control group (eGFP A: p = 0.0045; eGFP B: p = 0.0045). The expression levels of mice in the 1.52 MPa group was significantly higher than those of the other two groups for eGFP A and eGFP B (eGFP A: p = 0.012; eGFP B: p = 0.012).