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. 2018 Apr 26;8:6553. doi: 10.1038/s41598-018-24516-7

Table 1.

Forward and reverse primers used in qPCR for eGFP mRNA and 5.8S rRNA.

Primer Forward Reverse
eGFP A AGAACGGCATCAAGGTGAAC TGCTCAGGTAGTGGTTGTCG
eGFP B TATATCATGGCCGACAAGCA ACTGGGTGCTCAGGTAGTGG
5.8S rRNA GACTCTTAGCGGTGGATCACT CGTTCTTCATCGACGCACGA

Two primers were used for eGFP quantification, called eGFP A and eGFP B. 5.8S rRNA was used as an internal control.