Fig. 3. Manipulation of HOXA4 expression in lung cancer cell lines.

a Protein (top panel) and mRNA (bottom panel) expression of HOXA4 in 5 lung cancer cell lines and the BEAS-2B cell line. GAPDH was used as an internal control. b NCI-H1975 and NCI-H446 cells were infected with HOXA4-expressing lentivirus or control vector lentivirus (Vector), and HOXA4 protein (top panel) and mRNA (bottom panel) expression were analyzed at 48 h after viral infection. Wild-type cells (WT) served as a negative control. ***P < 0.001. c NCI-H1299 cells were infected with HOXA4 shRNA lentivirus (shHOXA4-1, 2, and 3) or control shRNA lentivirus (shNC). HOXA4 protein (top panel) and mRNA (bottom panel) expression were analyzed at 48 h post viral infection. ***P < 0.001 vs. shNC, ^#P < 0.05 vs. shHOXA4-1, ⁺⁺P < 0.01 vs. shHOXA4-2