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. 2018 Apr 27;9(5):465. doi: 10.1038/s41419-018-0497-x

Fig. 6. HOXA4 suppressed the Wnt signaling pathway in lung cancer cells.

Fig. 6

a, b Western blot analysis of GSK3β, β-catenin, Cyclin D1, c-Myc and Survivin in NCI-H1975 and NCI-H446 cells with HOXA4 overexpression (a) and NCI-H1299 cells with HOXA4 silencing (b). GAPDH was used as a loading control. c, d qRT-PCR analysis of GSK3β mRNA levels in NCI-H1975 and NCI-H446 cells with HOXA4 overexpression (c) and NCI-H1299 cells with HOXA4 silencing (d). e A luciferase reporter assay was performed to evaluate GSK3β promoter activity in NCI-H1975 and NCI-H446 cells with HOXA4 overexpression or silencing. f ChIP-qPCR for the GSK3β promoter in NCI-H1975 and NCI-H446 cells with HOXA4 overexpression or silencing. ***P < 0.001